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bal protein level  (Bio-Rad)


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    Bio-Rad bal protein level
    Murine lung infection with IAV is characterized by two distinct phases, a viral clearance phase and a lung injury phase, with white blood cell infiltration during former and latter phases. C57Bl/6 mice received intranasal administration of IAV H1N1 PR8 (800 pfu/mouse) on Day0. Lungs and <t>BAL</t> were collected on the days indicated to analyze for BAL total protein levels ( a ) and white blood cell count ( b ), lung wet/dry ratio ( c <t>),</t> <t>acellular</t> BAL granzyme B ( d ) and MPO ( e ) levels, and PR8 hemagglutinin level ( f ). n = 4–8 per group, presented are mean +/− SEM. * for p < 0.05 by t -test with Welch correction (unequal variances) between IAV groups and the control group, receiving saline in lung (plotted as D0).
    Bal Protein Level, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 43189 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bal+protein+levels/Protein+Standard+I/pmc08063825-74-0-11
    Average 99 stars, based on 43189 article reviews
    bal protein level - by Bioz Stars, 2026-10
    99/100 stars

    Images

    1) Product Images from "Concentrated Secretome of Adipose Stromal Cells Limits Influenza A Virus-Induced Lung Injury in Mice"

    Article Title: Concentrated Secretome of Adipose Stromal Cells Limits Influenza A Virus-Induced Lung Injury in Mice

    Journal: Cells

    doi: 10.3390/cells10040720

    Murine lung infection with IAV is characterized by two distinct phases, a viral clearance phase and a lung injury phase, with white blood cell infiltration during former and latter phases. C57Bl/6 mice received intranasal administration of IAV H1N1 PR8 (800 pfu/mouse) on Day0. Lungs and BAL were collected on the days indicated to analyze for BAL total protein levels ( a ) and white blood cell count ( b ), lung wet/dry ratio ( c ), acellular BAL granzyme B ( d ) and MPO ( e ) levels, and PR8 hemagglutinin level ( f ). n = 4–8 per group, presented are mean +/− SEM. * for p < 0.05 by t -test with Welch correction (unequal variances) between IAV groups and the control group, receiving saline in lung (plotted as D0).
    Figure Legend Snippet: Murine lung infection with IAV is characterized by two distinct phases, a viral clearance phase and a lung injury phase, with white blood cell infiltration during former and latter phases. C57Bl/6 mice received intranasal administration of IAV H1N1 PR8 (800 pfu/mouse) on Day0. Lungs and BAL were collected on the days indicated to analyze for BAL total protein levels ( a ) and white blood cell count ( b ), lung wet/dry ratio ( c ), acellular BAL granzyme B ( d ) and MPO ( e ) levels, and PR8 hemagglutinin level ( f ). n = 4–8 per group, presented are mean +/− SEM. * for p < 0.05 by t -test with Welch correction (unequal variances) between IAV groups and the control group, receiving saline in lung (plotted as D0).

    Techniques Used: Infection, Cell Counting, Control, Saline

    ASC-CS administration limits BAL protein and inflammatory cell extravasation, and increases in granzyme B and MPO without compromise of viral clearance in IAV-infected mice. C57Bl/6 mice received intranasal administration of IAV H1N1 PR8 (800 pfu/mouse) on Day0. Mice receiving vehicle control for IAV are annotated as saline (sal, black columns). On Day6 and 8, IAV mice were sub-cutaneously injected with 2.25 mg/kg of ASC-CS (CS, green columns) or an equivalent volume of PBS (red columns designated as IAV). Lungs and BAL were collected on Day9 to analyze for BAL white blood cell count ( a ) and total protein levels ( b ), lung wet/dry ratio ( c ), acellular BAL granzyme B ( d ) and MPO ( e ) levels, and PR8 hemagglutinin (HA) level ( f ). n = 4–6 per group, presented are mean +/− SEM. * for p < 0.05 by t -test with Welch correction (unequal variances) between (1) IAV groups and the control group (vs. sal), and (2) CM groups and the corresponding gender IAV group (vs. IAV).
    Figure Legend Snippet: ASC-CS administration limits BAL protein and inflammatory cell extravasation, and increases in granzyme B and MPO without compromise of viral clearance in IAV-infected mice. C57Bl/6 mice received intranasal administration of IAV H1N1 PR8 (800 pfu/mouse) on Day0. Mice receiving vehicle control for IAV are annotated as saline (sal, black columns). On Day6 and 8, IAV mice were sub-cutaneously injected with 2.25 mg/kg of ASC-CS (CS, green columns) or an equivalent volume of PBS (red columns designated as IAV). Lungs and BAL were collected on Day9 to analyze for BAL white blood cell count ( a ) and total protein levels ( b ), lung wet/dry ratio ( c ), acellular BAL granzyme B ( d ) and MPO ( e ) levels, and PR8 hemagglutinin (HA) level ( f ). n = 4–6 per group, presented are mean +/− SEM. * for p < 0.05 by t -test with Welch correction (unequal variances) between (1) IAV groups and the control group (vs. sal), and (2) CM groups and the corresponding gender IAV group (vs. IAV).

    Techniques Used: Infection, Control, Saline, Injection, Cell Counting

    Related Articles

    Bradford Assay:

    Article Title: Obesity Is Associated with Neutrophil Dysfunction and Attenuation of Murine Acute Lung Injury
    Article Snippet: IL-6, keratinocyte cytokine (KC), TNF-α, macrophage inflammatory protein (MIP)-2, and monocyte chemotactic protein (MCP)-1 levels in mouse plasma and BAL supernatants were assessed by Bio-Plex suspension-array system (Bio-Rad, Hercules, CA). .. BAL protein levels were measured by Bradford assay (Bio-Rad). ..

    Article Title: Obesity Is Associated with Neutrophil Dysfunction and Attenuation of Murine Acute Lung Injury
    Article Snippet: IL-6, keratinocyte cytokine (KC), TNF-a, macrophage inflammatory protein (MIP)-2, and monocyte chemotactic protein (MCP)-1 levels in mouse plasma and BAL supernatants were assessed by Bio-Plex suspension-array system (Bio-Rad, Hercules, CA). .. BAL protein levels were measured by Bradford assay (Bio-Rad). ..

    Article Title: Leptin downregulates LPS-induced lung injury: role of corticosterone and insulin.
    Article Snippet: .. BAL protein levels were measured by Bradford assay (Bio-Rad). .. Phenotypic analysis of lymphocyte subpopulations were assessed by three-color FACS using a FACScalibur Cytometer equipped with Cell Quest software (Becton and Dickinson, San Jose, CA, USA) with gates defined by forward and side light scattering properties.

    DC Protein Assay:

    Article Title: Dietary zinc mediates inflammation and protects against wasting and metabolic derangement caused by sustained cigarette smoke exposure in mice.
    Article Snippet: In mouse asthma models, inflammation can be modulated by zinc (Zn).. Given that appetite loss, muscle wasting and poor nutrition are features of chronic obstructive pulmonary disease (COPD) and that poor dietary Zn intake is in itself accompanied by growth retardation and appetite loss, we hypothesised that dietary Zn limitation would not only worsen airway inflammation but also exaggerate metabolic effects of cigarette smoke (CS) exposure in mice.. Conversely, Zn supplementation would lessen inflammation.

    Multiplex Assay:

    Article Title: Azithromycin and the treatment of lymphocytic airway inflammation after lung transplantation.
    Article Snippet: .. BAL protein levels were determined using a multiplex immunoassay (Bio-Plex ProTM human cytokine 27-plex assay; Bio-Rad Laboratories N.V., Nazareth, Belgium) according to manufacturer’s guidelines. ..



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    Murine lung infection with IAV is characterized by two distinct phases, a viral clearance phase and a lung injury phase, with white blood cell infiltration during former and latter phases. C57Bl/6 mice received intranasal administration of IAV H1N1 PR8 (800 pfu/mouse) on Day0. Lungs and <t>BAL</t> were collected on the days indicated to analyze for BAL total protein levels ( a ) and white blood cell count ( b ), lung wet/dry ratio ( c <t>),</t> <t>acellular</t> BAL granzyme B ( d ) and MPO ( e ) levels, and PR8 hemagglutinin level ( f ). n = 4–8 per group, presented are mean +/− SEM. * for p < 0.05 by t -test with Welch correction (unequal variances) between IAV groups and the control group, receiving saline in lung (plotted as D0).
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    Murine lung infection with IAV is characterized by two distinct phases, a viral clearance phase and a lung injury phase, with white blood cell infiltration during former and latter phases. C57Bl/6 mice received intranasal administration of IAV H1N1 PR8 (800 pfu/mouse) on Day0. Lungs and <t>BAL</t> were collected on the days indicated to analyze for BAL total protein levels ( a ) and white blood cell count ( b ), lung wet/dry ratio ( c <t>),</t> <t>acellular</t> BAL granzyme B ( d ) and MPO ( e ) levels, and PR8 hemagglutinin level ( f ). n = 4–8 per group, presented are mean +/− SEM. * for p < 0.05 by t -test with Welch correction (unequal variances) between IAV groups and the control group, receiving saline in lung (plotted as D0).
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    Image Search Results


    Murine lung infection with IAV is characterized by two distinct phases, a viral clearance phase and a lung injury phase, with white blood cell infiltration during former and latter phases. C57Bl/6 mice received intranasal administration of IAV H1N1 PR8 (800 pfu/mouse) on Day0. Lungs and BAL were collected on the days indicated to analyze for BAL total protein levels ( a ) and white blood cell count ( b ), lung wet/dry ratio ( c ), acellular BAL granzyme B ( d ) and MPO ( e ) levels, and PR8 hemagglutinin level ( f ). n = 4–8 per group, presented are mean +/− SEM. * for p < 0.05 by t -test with Welch correction (unequal variances) between IAV groups and the control group, receiving saline in lung (plotted as D0).

    Journal: Cells

    Article Title: Concentrated Secretome of Adipose Stromal Cells Limits Influenza A Virus-Induced Lung Injury in Mice

    doi: 10.3390/cells10040720

    Figure Lengend Snippet: Murine lung infection with IAV is characterized by two distinct phases, a viral clearance phase and a lung injury phase, with white blood cell infiltration during former and latter phases. C57Bl/6 mice received intranasal administration of IAV H1N1 PR8 (800 pfu/mouse) on Day0. Lungs and BAL were collected on the days indicated to analyze for BAL total protein levels ( a ) and white blood cell count ( b ), lung wet/dry ratio ( c ), acellular BAL granzyme B ( d ) and MPO ( e ) levels, and PR8 hemagglutinin level ( f ). n = 4–8 per group, presented are mean +/− SEM. * for p < 0.05 by t -test with Welch correction (unequal variances) between IAV groups and the control group, receiving saline in lung (plotted as D0).

    Article Snippet: BAL protein level was measured in acellular BAL using Bradford assay (BioRad, Hercules, CA, USA).

    Techniques: Infection, Cell Counting, Control, Saline

    ASC-CS administration limits BAL protein and inflammatory cell extravasation, and increases in granzyme B and MPO without compromise of viral clearance in IAV-infected mice. C57Bl/6 mice received intranasal administration of IAV H1N1 PR8 (800 pfu/mouse) on Day0. Mice receiving vehicle control for IAV are annotated as saline (sal, black columns). On Day6 and 8, IAV mice were sub-cutaneously injected with 2.25 mg/kg of ASC-CS (CS, green columns) or an equivalent volume of PBS (red columns designated as IAV). Lungs and BAL were collected on Day9 to analyze for BAL white blood cell count ( a ) and total protein levels ( b ), lung wet/dry ratio ( c ), acellular BAL granzyme B ( d ) and MPO ( e ) levels, and PR8 hemagglutinin (HA) level ( f ). n = 4–6 per group, presented are mean +/− SEM. * for p < 0.05 by t -test with Welch correction (unequal variances) between (1) IAV groups and the control group (vs. sal), and (2) CM groups and the corresponding gender IAV group (vs. IAV).

    Journal: Cells

    Article Title: Concentrated Secretome of Adipose Stromal Cells Limits Influenza A Virus-Induced Lung Injury in Mice

    doi: 10.3390/cells10040720

    Figure Lengend Snippet: ASC-CS administration limits BAL protein and inflammatory cell extravasation, and increases in granzyme B and MPO without compromise of viral clearance in IAV-infected mice. C57Bl/6 mice received intranasal administration of IAV H1N1 PR8 (800 pfu/mouse) on Day0. Mice receiving vehicle control for IAV are annotated as saline (sal, black columns). On Day6 and 8, IAV mice were sub-cutaneously injected with 2.25 mg/kg of ASC-CS (CS, green columns) or an equivalent volume of PBS (red columns designated as IAV). Lungs and BAL were collected on Day9 to analyze for BAL white blood cell count ( a ) and total protein levels ( b ), lung wet/dry ratio ( c ), acellular BAL granzyme B ( d ) and MPO ( e ) levels, and PR8 hemagglutinin (HA) level ( f ). n = 4–6 per group, presented are mean +/− SEM. * for p < 0.05 by t -test with Welch correction (unequal variances) between (1) IAV groups and the control group (vs. sal), and (2) CM groups and the corresponding gender IAV group (vs. IAV).

    Article Snippet: BAL protein level was measured in acellular BAL using Bradford assay (BioRad, Hercules, CA, USA).

    Techniques: Infection, Control, Saline, Injection, Cell Counting